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1,4‐Dihydropyridine receptor associated with Ca 2+ channels in human embryonic fibroblasts
Author(s) -
Dudkin Sergei M.,
Gnedoj Sergei N.,
Chernyuk Natalia N.,
Soldatov Nikolai M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80458-7
Subject(s) - embryonic stem cell , receptor , microbiology and biotechnology , chemistry , dihydropyridine , biophysics , biology , biochemistry , calcium , gene , organic chemistry
By using the radioactively labeled 1,4‐dihydropyridine (DHP) probe, [ 3 H]PMD, we have demonstrated that cultured human embryonic fibroblasts grown at a low density in Eagle's medium supplemented with serum contain a single class of non‐interacting DHP binding sites ( B max , 1.2±0.3 pmol/ 10 6 cells; K d , 3.9 nM). After inhibition of the DHP receptor biosynthesis by cycloheximide, the number of [ 3 H]PMD binding sites is reduced with a half‐time of 12 h, which implies a turnover rate of 30 000±7500 receptors/h per cell. With progression to confluency, the B max value decreased up to 0.28±0.08 pmol/10 6 cells without significant change in K d value. When cells were grown at a low density in serum‐free conditions, the number of [ 3 H]PMD binding sites gradually increased 1.9‐fold within 3 days. Addition of serum reversed this effect with the same time course. These results imply that the DHP‐sensitive Ca 2+ channels are involved in the control of the proliferation of human embryonic fibroblasts.