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Insulin‐like growth factor 1 and insulin reduce epidermal growth factor binding to Swiss 3T3 cells by an indirect mechanism that is apparently independent of protein kinase C
Author(s) -
Corps Anthony N.,
Brown Kenneth D.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80447-2
Subject(s) - epidermal growth factor , insulin , protein kinase c , insulin receptor , protein kinase a , growth factor , insulin like growth factor , endocrinology , medicine , irs2 , chemistry , biology , kinase , biochemistry , receptor , insulin resistance
Insulin‐like growth factor 1 and insulin reduced the binding of 125 I‐labelled epidermal growth factor ( 125 I‐EGF) to Swiss 3T3 cells by 15–20% at 37°C, but not at 4°C. Scatchard analysis indicated that IGF‐1 and insulin affected the higher‐affinity component of EGF binding, an effect previously associated with the activation of protein kinase C. However, the inhibition of 125 I‐EGF binding by IGF‐1 and insulin was increased, not reduced, when the cells were treated with high concentrations of phorbol esters to down‐modulate protein kinase C. We suggest that IGF‐1 and insulin activate a protein kinase with similar or overlapping specificity to that of protein kinase C.