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Increased levels of acetylcholine receptor α‐subunit mRNA in experimental autoimmune myasthenia gravis
Author(s) -
Asher Orna,
Neumann Drorit,
Fuchs Sara
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80442-3
Subject(s) - myasthenia gravis , acetylcholine receptor , messenger rna , protein subunit , endocrinology , acetylcholine , receptor , biology , complementary dna , medicine , microbiology and biotechnology , immunology , gene , biochemistry
To gain insight into the regulatory mechanisms underlying the blockade and loss of acetylcholine receptor (AChR) in myasthenia, we have followed AChR α‐subunit mRNA levels in leg muscles of myasthenic and normal rabbits and rats. Northern blots of RNA preparations from normal and myasthenic animals were hybridized with a mouse AChR α‐subunit cDNA probe. Our experiments indicate a specific increase (4–7‐fold) in the levels of α‐subunit mRNA in animals with experimental autoimmune myasthenia gravis (EAMG), in comparison with control animals. Actin mRNA levels were essentially unchanged. Our results thus suggest that EAMG is accompanied by an increased level of AChR gene transcription.