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Activation of c‐Ki‐ ras coexists with c‐ myc amplification in cells from a nude mouse tumor induced by the human breast carcinoma cell line SW 613‐S
Author(s) -
Carloni Guido,
Champ Brigitte,
Vilarem Marie-Jose´,
Lavialle Christian,
Cassingena Roland
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80440-x
Subject(s) - transfection , cell culture , phenotype , microbiology and biotechnology , biology , gene , cancer research , in vitro , nude mouse , 3t3 cells , cell , genetics
In vitro transfection experiments have shown that cooperation between two different oncogenes can confer a fully malignant phenotype to primary rodent cells. We have previously reported that SW 613‐Tul cells, derived from a tumor induced in a nude mouse by the human breast carcinoma cell line SW 613‐S, showed a 30‐fold amplification of the c‐ myc gene. In the present work, we show that these cells also harbor an activated c‐Ki‐ ras gene capable of inducing the formation of foci upon transfection of NIH 3T3 cells with SW 613‐Tul genomic DNA. Our results suggest that both the c‐ myc and c‐Ki‐ ras oncogenes, activated by two different mechanisms, may cooperate in the full expression of the tumorigenic phenotype of SW 613‐Tul cells.