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Posttranscriptional regulation of the expression of CAD gene during differentiation of F9 teratocarcinoma cells by induction with retinoic acid and dibutyryl cyclic AMP
Author(s) -
Rao Gadiparthi N.,
Church Robert L.,
Davidson Jeffrey N.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80424-1
Subject(s) - retinoic acid , teratocarcinoma , microbiology and biotechnology , retinoic acid inducible orphan g protein coupled receptor , chemistry , gene expression , retinoic acid receptor gamma , retinoic acid receptor , cellular differentiation , gene , biology , biochemistry
We have studied the regulation of expression of the carbamoyl‐phosphate synthetase II‐aspartate transcarbamylase‐dihydroorotase gene in F9 teratocarcinoma cells during their differentiation into parietal endoderm cells by induction with a combination of retinoic acid and dibutyryl cyclic AMP. Steady‐state levels of CAD mRNA decreased by 7‐fold in F9 cells following 120 h of retinoic acid and dibutyryl cyclic AMP induction as compared to levels in uninduced cells. Conversely, no apparent changes were found in the steady‐state levels of β‐actin mRNA between induced and uninduced cells. Despite a 7‐fold decrease in the steady‐state levels of CAD mRNA, its rate of transcription remained the same between induced and uninduced cells, indicating a role for posttranscriptional mechanisms for its down regulation during retinoic acid‐ and dibutyryl cyclic AMP‐induced differentiation of F9 cells. The cellular growth rate of F9 cells as determined by [ 3 H]thymidine uptake and parallel cell counting decreased markedly during their induction with retinoic acid and dibutyryl cyclic AMP. Taken together, it is apparent that the expression of the CAD gene is cell‐growth‐dependent and its regulation in this system is at the posttranscriptional level.