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Strand‐specific transcription of the simple sequences poly[(dG‐dT):(dC‐dA)] and poly[(dG‐dA):(dC‐dT)] in D. melanogaster embryos revealed by S 1 nuclease treatment
Author(s) -
Vashakidze R.P.,
Mamulashvili N.A.,
Kalandarishvili K.G.,
Kolchinsky A.M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80423-x
Subject(s) - nuclease , chemistry , microbiology and biotechnology , transcription (linguistics) , biology , biophysics , dna , biochemistry , linguistics , philosophy
The transcription of different strands of the simple sequences poly[(dG‐dT):(dC‐dA)] and poly[(dG‐dA):(dT‐dC)] in Drosophila melanogaster embryos was studied. After selective labelling of one strand, the polymers were hybridized with immobilized RNA and hybrids were treated on filters with S 1 nuclease. Appropriate control has shown that this treatment destroys sandwich‐like complexes and leaves only true hybrids. Transcription of poly[(dG‐dA):(dT‐dC)] proved to be nearly symmetric, while poly(dG‐dT) is transcribed 3‐times more intensively than poly(dA‐dC). Our approach allowed us to estimate the relative content of simple sequences in RNA.