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The γ‐subunit of ATP synthase from spinach chloroplasts Primary structure deduced from the cloned cDNA sequence
Author(s) -
Miki Junji,
Maeda Masatomo,
Mukohata Yasuo,
Futai Masamitsu
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80421-6
Subject(s) - chloroplast , protein subunit , complementary dna , atp synthase , protein primary structure , biology , peptide sequence , biochemistry , atp synthase gamma subunit , microbiology and biotechnology , spinach , nucleic acid sequence , oligonucleotide , cdna library , dna , gene , enzyme , atpase , atp hydrolysis
cDNA clones encoding the γ‐subunit of chloroplast ATP synthase were isolated from a spinach library using synthetic oligonucleotide probes. The predicted amino acid sequence indicated that the mature chloroplast γ‐subunit consists of 323 amino acid residues and is highly homologous (55% identical residues) with the sequence of the cyanobacterial subunit. The positions of the four cysteine residues were identified. The carboxyl‐terminal region of the choloroplast γ‐subunit is highly homologous with those of the γ‐subunits from six other sources (bacteria and mitochondria) sequenced thus far.