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Escherichia coli hemolysin permeabilizes small unilamellar vesicles loaded with calcein by a single‐hit mechanism
Author(s) -
Menestrina Gianfranco
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80420-4
Subject(s) - calcein , vesicle , biophysics , escherichia coli , chemistry , valinomycin , liposome , diphtheria toxin , hemolysin , cooperativity , membrane , biochemistry , toxin , biology , virulence , gene
Escherichia coli hemolysin produces small unilamellar lipid vesicles permeable to the fluorescent dye calcein by forming pores through their membrane. The process of permeabilization proceeds as a pseudo first‐order reaction, indicating that the toxin is active as a monomer; consistently no evidence for cooperativity has been found in a dose‐response titration. The rate of interaction increases on lowering the pH of the solution and by introducing negatively charged lipids into the vesicles. The overall pore formation mechanism resembles that of other toxins of bacterial origin such as colicins, diphtheria, tetanus and botulinum toxin.