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Mutual inactivation of valinomycin and protonophores by complex formation in liposomal membranes
Author(s) -
Krishnamoorthy G.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80416-2
Subject(s) - liposome , valinomycin , membrane , chemistry , biophysics , biochemistry , biology
The simultaneous presence of a protonophore [3,5‐di( tert ‐butyl)‐4‐hydroxybenzylidenemalononitrile or carbonyl cyanide m ‐chlorophenylhydrazone] and valinomycin in a liposome suspension results in time‐dependent inactivation of ion transport by both the protonophore and valinomycin. Correlation of the inactivation with spectrophotometric observations on the formation of a complex between the protonophore and valinomycin strongly suggests that the complex observed has no (or very low) activity for the transport of either H + or K + . The stoichiometry of valinomycin and the protonophore in the inactive complex is shown to be 1:1.