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Initial and sustained calcium mobilizations in the parietal cell during stimulations with gastrin, inositol trisphosphate, phorbol ester and erogenous diacylglycerol
Author(s) -
Tsunoda Yasuhiro,
Takeda Hiroshi,
Asaka Masahiro,
Nakagaki Ikuko,
Sasaki Sadao
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80391-0
Subject(s) - diacylglycerol kinase , gastrin , inositol , extracellular , aequorin , calcium , medicine , fura 2 , intracellular , chemistry , endocrinology , cytoplasm , second messenger system , protein kinase c , biology , biochemistry , receptor , secretion , phosphorylation , cytosol , enzyme
Electron probe X‐ray microanalysis revealed that cytoplasmic Ca 2+ concentration increased in the restricted apical cytoplasm during stimulation of isolated guinea pig parietal cells with gastrin. Furthermore, this study, using 45 Ca 2+ , aequorin and fura‐2, revealed the mechanism involved in intracellular Ca 2+ shifts caused by gastrin and the involvements of inositol 1,4,5‐trisphosphate (IP 3 ) and diacylglycerol in producing those shifts. The gastrin‐mediated and IP 3 ‐sensitive Ca 2+ pool was located in the smooth‐surfaced membrane‐enriched areas and released Ca 2+ in the initial phase. Gastrin‐mediated Ca 2+ mobilization was also evoked by diacylglycerol, comprising an intracellular Ca 2+ mobilization followed by a late, sustained and more localised Ca 2+ entry from the extracellular space.