Premium
Tumor necrosis factor and interleukin 1 activate phospholipase in rat chondrocytes
Author(s) -
Suffys Philip,
Van Roy Frans,
Fiers Walter
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80379-x
Subject(s) - tumor necrosis factor alpha , chondrocyte , cytokine , microbiology and biotechnology , interleukin , phospholipase a2 , phospholipase , chemistry , macrophage , phospholipase a , biology , endocrinology , immunology , medicine , biochemistry , enzyme , in vitro
Tumor necrosis factor (TNF) and interleukin 1 (IL‐1) are both cytokines of macrophage origin with similar activity on several cell types. We investigated whether TNF can, analogously to IL‐1, stimulate phospholipase activity of chondrocytes. Addition of each of these cytokines to cells, isolated from the xiphisternum of adult rats, resulted in a time‐ and dose‐dependent increase in phospholipase activity in both secreted and membrane‐associated form. Moreover, TNF and IL‐1 both induce a transformation of chondrocyte morphology. In conclusion, TNF stimulates chondrocyte phospholipase activity and extends the long list of actions shared by IL‐1 and TNF in a diversity of cellular systems.