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Two isoforms of protein phosphatase 1 may be produced from the same gene
Author(s) -
Cohen Patricia T.W.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80378-8
Subject(s) - phosphatase , complementary dna , microbiology and biotechnology , gene isoform , gene , peptide sequence , dusp6 , protein phosphatase 1 , biology , protein subunit , biochemistry , protein phosphatase 2 , cdna library , nucleic acid sequence , enzyme
A cDNA clone encoding a second type‐1 protein phosphatase catalytic subunit (1α) was isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The deduced protein sequence (330 residues, 37.5 kDa) was 19 residues longer at its N‐terminus than protein phosphatase 1β (311 residues, 35.4 kDa). The amino acid sequences of protein phosphatases 1α and 1β were identical after residue 33 of protein phosphatase 1α. The results suggest that the different N‐terminal sequences of protein phosphatases 1α and 1β are likely to be generated by differential transcription or processing of the mRNA transcribed from a single gene. Southern blotting of rabbit DNA was consistent with this interpretation.