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Effect of Graves' IgG on gene transcription in human thyroid cell cultures Thyroglobulin gene activation
Author(s) -
Kung Annie W.C.,
Collison Kate,
Banga J.Paul,
McGregor Alan M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80377-6
Subject(s) - thyroglobulin , medicine , endocrinology , thyroid , messenger rna , gene expression , antibody , graves' disease , hormone , stimulation , gene , biology , chemistry , immunology , biochemistry
In Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitative activation of thyroglobulin (TG) gene in cultured human thyroid cells by RNA hybridisation. TG mRNA expression was activated by TSH and Graves' IgG. Nuclear transcription assays showed that the increase in cytoplasmic mRNA levels was due to increased transcription of TG specific mRNA in nuclei of thyroid cells. Whilst TSH led to a dose dependent increase in TG mRNA levels, Graves' IgG led to a variable activation of TG gene. A significant correlation between the increased TG mRNA transcription and cAMP production was observed with Graves' IgG. Thus the activation of the TG gene by Graves' IgG occurs in parallel with elevation of cAMP.