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Phorbol ester‐responsive H‐ ras 1 gene promoter contains multiple TPA‐inducible/AP‐1‐binding consensus sequence elements
Author(s) -
Spandidos Demetrios A.,
Nichols Rosely A.B.,
Wilkie Neil M.,
Pintzas Alex
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80366-1
Subject(s) - microbiology and biotechnology , plasmid , transfection , gene , recombinant dna , biology , promoter , mutant , transcription (linguistics) , gene expression , biochemistry , linguistics , philosophy
We have constructed recombinant DNA plasmids which carry both the aminoglycoside phosphotransferase (aph) gene and the chloramphenicol acetyl‐transferase (CAT) gene linked to the human normal or mutant T24 H‐ ras 1 promoter. We have transfected these plasmids into rat 208F fibroblasts using the calcium phosphate technique and selected for stable transformants by geneticin resistance. These transformants expressed CAT activity at low levels. However, when treated with the phorbol ester TPA, CAT levels increased substantially. Cells transfected with recombinant plasmids carrying a promoterless CAT gene did not respond to TPA. We have noted four motifs in the H‐ ras 1 promoter region which resemble TPA‐inducible and AP‐1‐binding consensus sequences. We suggest that AP‐1‐like proteins may play a role in control of H‐ ras 1 transcription.