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Generation of phosphatidylinositol 4,5‐bisphosphate proceeds through an intracellular route in rat hepatocytes
Author(s) -
Lundberg Gunilla A.,
Jergil Bengt
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80362-4
Subject(s) - phosphatidylinositol 4,5 bisphosphate , phosphatidylinositol , intracellular , microbiology and biotechnology , chemistry , biochemistry , biophysics , biology , signal transduction
The subcellular distribution in rat hepatocytes of enzymes participating in the entire generation cycle of phosphatidylinositol 4,5‐bisphosphate, and phosphorylated intermediates of this pathway, has been examined by Nycodenz gradient centrifugation. Our results indicate that the synthesis of phosphatidylinositol takes place in the endoplasmic reticulum, and that its phosphorylation to phosphatidylinositol 4‐phosphate occurs intracellularly in low‐density membranes before translocation to the plasma membrane, where it is further phosphorylated to phosphatidylinositol 4,5‐bisphosphate. The intracellular formation of PIP implies a vesicular transport to the plasma membrane.