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Inhibition by amiloride and by Na + ‐depletion of A23187‐stimulated arachidonic acid and histamine release from rat mast cells
Author(s) -
Linnebjerg Helle,
Hansen Harald S.,
Jensen Benny
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80361-2
Subject(s) - arachidonic acid , histamine , chemistry , amiloride , extracellular , phospholipase a2 , phosphatidylcholine , mast cell , calcium , intracellular , biochemistry , endocrinology , phospholipid , enzyme , biology , sodium , membrane , immunology , organic chemistry
Rat peritoneal mast cells, labelled with [ 14 C]arachidonic acid, released histamine and [ 14 C]arachidonic acid upon the addition of A23187. The release induced by low concentrations of A23187 was suppressed by removal of extracellular Na + and by addition of the Na + /H + exchange inhibitor, amiloride. Addition of protein kinase C inhibitors resulted in only a modest decrease of [ 14 C]arachidonic acid and histamine release. [ 14 C]Arachidonic acid was hydrolyzed from both phosphatidylcholine and triacylglycerol. Amiloride inhibited only the hydrolysis from phosphatidylcholine. It is suggested that an Na + /H + exchange is stimulated secondary to an A23187‐induced increase in intracellular calcium concentration. Increased Na + /H + exchange seems to facilitate the activation by intracellular calcium ions of phospholipase A 2 as well as of histamine secretion.