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Reconstitution of plastoquinone in the D1/D2/cytochrome b ‐559 photosystem II reaction centre complex
Author(s) -
Gounaris Kleoniki,
Chapman David J.,
Barber James
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80356-9
Subject(s) - plastoquinone , photosystem ii , cytochrome b , chemistry , cytochrome b6f complex , photochemistry , cytochrome , photosystem i , photosynthetic reaction centre , electron transfer , biochemistry , photosynthesis , enzyme , chloroplast , mitochondrial dna , thylakoid , gene
Reconstitution of plastoquinone in the photosystem II D1/D2/cytochrome b ‐559 reaction centre complex, in the presence of the detergent Triton X‐100, is reported. Illumination of the reconstituted system results in the reduction of cytochrome b ‐559, the process being partly herbicide‐sensitive. In addition, the reconstitution of plastoquinone results in the ability of the isolated reaction centre to catalyse the photoreduction of 2,6‐dichlorophenolindophenol in the presence of the exogenous electron donor diphenylcarbazide.