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Fractionation of synaptophysin‐containing vesicles from rat brain and cultured PC12 pheochromocytoma cells
Author(s) -
Wiedenmann Bertram,
Rehm Hubert,
Knierim Martin,
Becker Cord-Michael
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80342-9
Subject(s) - synaptophysin , synaptic vesicle , vesicle , adrenal medulla , chemistry , microbiology and biotechnology , biology , endocrinology , membrane , biochemistry , immunohistochemistry , catecholamine , immunology
Synaptophysin is a transmembrane glycoprotein of neuroendocrine vesicles. Its content and distribution in subcellular fractions from cultured PC12 cells, rat brain and bovine adrenal medulla were determined by a sensitive dot immunoassay. Synaptophysin‐containing fractions appeared as monodispersed populations similar to synaptic vesicles in density and size distribution. Membranes from synaptic vesicles contained ≈ 100‐times more synaptophysin than chromaffin granules. In conclusion, synaptophysin is located almost exclusively in vesicles of brain and PC12 cells which are distinct from dense core granules.