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ATP stimulates the uptake of S ‐dinitrophenylglutathione by rat liver plasma membrane vesicles
Author(s) -
Kobayashi Kazuo,
Sogame Yoshihisa,
Hayashi Koichiro,
Nicotera Pierluigi,
Orrenius Sten
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80339-9
Subject(s) - glutathione , vesicle , glutathione disulfide , chemistry , incubation , biochemistry , oxidative phosphorylation , membrane , biophysics , biology , enzyme
Incubation of inverted plasma membrane vesicles from rat liver with micromolar concentrations of S ‐dinitrophenylglutathione (DNP‐SG) in the presence of ATP resulted in the uptake of DNP‐SG into the vesicles. ATP‐dependent DNP‐SG accumulation was half‐maximal with 9 μM DNP‐SG, while the K m for ATP was 320 μM. Glutathione disulfide (GSSG), but not reduced glutathione, inhibited the ATP‐dependent accumulation of DNP‐SG by the vesicles, suggesting that the same, ATP‐dependent transport system is responsible for the extrusion of glutathione conjugates and GSSG from liver cells.