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Occurrence and spacing of ribosome recognition sites in mRNAs of chloroplasts from higher plants
Author(s) -
Ruf Michael,
Kössel Hans
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80336-3
Subject(s) - ribosome , base pair , ribosomal rna , biology , stem loop , nucleotide , genetics , messenger rna , internal ribosome entry site , shine dalgarno sequence , rna , computational biology , gene
A computer‐aided search for potential ribosome recognition sequences of mRNAs from tobacco chloroplasts shows that more than 90% of mRNA species contain sequences upstream of the respective initiator codons, which allow base pairing with 3′‐terminal sequences of small subunit rRNA. This complementarity in several cases involves 16 S rRNA sequences between the canonical CCUCC sequence and the 3′‐terminal stem/loop structure. The distances between potential ribosome recognition sequences and initiator codons can be up to 25 nucleotides which is much greater when compared to the spacing of 7±2 nucleotides observed for the classical Shine‐Dalgarno sequences in bacterial mRNAs.