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COOH‐terminal residues of D1 and the 44 kDa CPa‐2 at spinach photosystem II core complex
Author(s) -
Takahashi Masaaki,
Shiraishi Takuo,
Asada Kozi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80330-2
Subject(s) - photosystem ii , spinach , chemistry , biochemistry , agarose , peptide , peptide sequence , stereochemistry , n terminus , microbiology and biotechnology , biology , gene , photosynthesis
The COOH‐termini of the 32 kDa D1 and 44 kDa CPa‐2 were determined by protein sequencing of peptides from trypsinized photosystem II core complexes. COOH‐terminal fragments were isolated by affinity chromatography using anhydrotrypsin‐agarose. One peptide had a sequence corresponding to the segment from Asn at position 335 to Ala at position 344 of the sequence deduced from the psb A gene coding for D1. Nine amino acids may be cleaved from the COOH‐terminus of pre‐D1 during maturation. In contrast, CPa‐2 was not modified at its COOH‐terminus.