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Hydrogen evolution by subchloroplast preparations of photosystem II from pea and spinach
Author(s) -
Mal'tsev Sergey V.,
Allakhverdiev Suleyman I.,
Klimov Vyacheslav V.,
Krasnovsky Alexander A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80329-6
Subject(s) - chemistry , photosystem ii , spinach , dcmu , atrazine , photochemistry , hydrogen , photosystem i , photosynthesis , biochemistry , organic chemistry , biology , pesticide , agronomy
Hydrogen (H 2 ) evolution rates were measured by the gas chromatographic technique upon illumination of different subchloroplast preparations of higher plants without exogenous hydrogenase under anaerobic conditions. Subchloroplast preparations enriched in photosystem II (PS II) in the presence of an electron donor TMPD ( N,N,N′,N′ ‐tetramethyl‐ p ‐phenylenediamine) are shown to have higher H 2 ‐evolution rates (up to 30 nmol/mg Chl per h) than preparations enriched in PS I under the same conditions. The data on the suppression of H 2 evolution by well‐known inhibitors of PS II (dinoseb, atrazine) prove that the H 2 photoproduction is sensitized by PS II reaction centers.