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31 P NMR study of daunorubicin‐d(CGTACG) complex in solution Evidence of the intercalation sites
Author(s) -
Ragg E.,
Mondelli R.,
Battistini C.,
Garbesi A.,
Colonna F.P.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80320-x
Subject(s) - intercalation (chemistry) , daunorubicin , chemistry , crystallography , inorganic chemistry , medicine , leukemia
The interaction of daunorubicin with the self‐complementary DNA fragment d(CGTACG) was studied by 31 P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 19°C were detected. In solution, the hexanucleotide binds two molecules of daunorubicin, which intercalate in the d(CG) sequence at both ends of the helix. Evidence for local deformations of the backbone at the sites of C 5 pG 6 , C 1 pG 2 and G 2 pT 3 phosphates is given. The binding constants for the stepwise equilibrium and the rate of dissociation of the intercalated duplex were also determined.