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Purification of fusion proteins expressed by pEX3 and a truncated pEX3 derivative
Author(s) -
Kocken Clemens H.M.,
Scheer J.M.José,
Welling Gjalt W.,
Welling-Wester Sytske
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80300-4
Subject(s) - fusion protein , biology , protein expression , amino acid , biochemistry , computational biology , recombinant dna , gene
A derivative of the pEX3 expression vector was constructed that codes for the first 407 amino acids of the 1051 amino acids of the pEX3 fusion protein. The amount of truncated fusion protein (40 mg/g cells), obtained by expression in E. coli , was similar to that produced by the original pEX3 vector. The truncated fusion protein was purified more easily from E. coli contaminants than the original fusion protein by washing with 2 M urea and 0.5% Triton X‐100.