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The muscarinic receptor subtype in mouse pancreatic B‐cells
Author(s) -
Henquin J.C.,
Nenquin M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80290-4
Subject(s) - pirenzepine , muscarinic acetylcholine receptor , muscarinic acetylcholine receptor m2 , muscarinic acetylcholine receptor m3 , muscarinic acetylcholine receptor m1 , receptor , endocrinology , medicine , muscarinic acetylcholine receptor m5 , atropine , muscarinic acetylcholine receptor m4 , acetylcholine , chemistry , acetylcholine receptor , biology , pharmacology
Isolated mouse islets were used to identify the muscarinic receptor subtype present in pancreatic B‐cells. We thus compared the inhibitory potencies of atropine (non‐specific), of pirenzepine (specific for M 1 receptors) and of compound AF‐DX 116 (specific for cardiac M 2 receptors) on acetylcholine‐induced insulin release, 86 Rb + efflux and 45 Ca 2+ efflux. The three antagonists inhibited all effects of acetylcholine, but EC 50 values were markedly different: atropine = 1.5–5 nM, pirenzepine = 0.6–1.7 μM and AF‐DX 116 = 1.7–11 μM. The results did not suggest that the various effects of ACh could result from the activation of different subtypes of receptors. It is concluded that muscarinic receptors of pancreatic B‐cells belong to an M 2 subtype distinct from the cardiac M 2 receptors.