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N‐terminal sequence analysis of the 8 kDa protein in Chlamydomonas reinhardii Localization of the phosphothreonine
Author(s) -
Dedner Norbert,
Meyer Helmut E.,
Ashton Chris,
Wildner Günter F.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80288-6
Subject(s) - edman degradation , threonine , biochemistry , protein sequencing , peptide sequence , amino acid , residue (chemistry) , chlamydomonas , sequence analysis , microbiology and biotechnology , biology , chemistry , phosphorylation , gene , serine , mutant
A phosphorylated 8 kDa protein of Chlamydomonas reinhardii thylakoids has been isolated and its N‐terminal amino acid sequence determined by gas‐phase sequencing. The sequence analysis of the 48 amino acid residues revealed that this protein is about 50% homologous to the psb H gene products of higher plants. In contrast to them, it contains an insert of seven amino acid residues (Ser‐5 to Lys‐11). The first threonine residue was phosphorylated as determined by 32 P detection during sequencing and also by analysis of the modified degradation products in the chemical reaction of the Edman degradation process. This latter method allows the identification of phosphorylated threonine residues without radiolabelling the protein.