Premium
Isolation and characterization of the porcine nuclear factor I (NFI) gene
Author(s) -
Meisterernst Michael,
Rogge Lars,
Donath Cornelia,
Gander Irene,
Lottspeich Friedrich,
Mertz Ronald,
Dobner Thomas,
Föckler Renate,
Stelzer Gertraud,
Winnacker Ernst-L.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80279-5
Subject(s) - isolation (microbiology) , gene , biology , genetics , chemistry , microbiology and biotechnology
This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5′‐flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen‐bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.