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Colchicine acts as a progression factor to initiate DNA synthesis in quiescent Balb/c 3T3 cells
Author(s) -
Shinohara Yukiko,
Nishida Eisuke,
Sakai Hikoichi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80277-1
Subject(s) - dna synthesis , colchicine , platelet derived growth factor receptor , growth factor , 3t3 cells , epidermal growth factor , fibroblast growth factor , platelet derived growth factor , cancer research , biology , microbiology and biotechnology , endocrinology , dna , chemistry , cell culture , biochemistry , receptor , genetics , transfection
In quiescent Balb/c 3T3 cells, competence factors such as 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA) and platelet‐derived growth factor (PDGF) synergize with progression factors such as insulin to initiate DNA synthesis.In this study, we found that colchicine, a microtubule‐disrupting agent, acted synergistically with TPA, but not with insulin, to induce the maximal stimulation of DNA synthesis. Colchicine also synergized with PDGF in the presence of epidermal growth factor to elicit nearly the optimal induction of DNA synthesis. Moreover, it acted synergistically with fibroblast growth factor, another competence factor. These results suggest that colchicine acts as a progression factor like insulin in quiescent Balb/c 3T3 cells.