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A major role for protein kinase C in calcium‐activated exocytosis in permeabilised adrenal chromaffin cells
Author(s) -
Burgoyne Robert D.,
Morgan Alan,
O'Sullivan Antony J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80246-1
Subject(s) - chromaffin cell , exocytosis , microbiology and biotechnology , protein kinase c , protein kinase a , chemistry , secretion , calcium , endogeny , kinase , biology , adrenal medulla , biochemistry , catecholamine , endocrinology , organic chemistry
The role of endogenously activated protein kinase C in calcium‐activated exocytosis was examined in digitonin‐permeabilised bovine adrenal chromaffin cells. Protein kinase C activity was reduced by down‐regulation following long‐term treatment with PMA or by using the inhibitor sphingosine. Both treatments resulted in a substantial reduction in catecholamine secretion elicited by micromolar calcium, indicating that endogenous activation of protein kinase C is a major requirement for calcium‐activated exocytosis in chromaffin cells.