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A nuclear yeast gene (GCY) encodes a polypeptide with high homology to a vertebrate eye lens protein
Author(s) -
Oechsner Ulrich,
Magdolen Viktor,
Bandlow Wolfhard
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80240-0
Subject(s) - biology , gene , homology (biology) , genetics , yeast , nuclear protein , transcription factor
We describe here the nuclear gene for a yeast protein showing unexpectedly high homology with mammalian aldo/keto reductases as well as with ϱ‐crystallin, one of the prominent proteins of the frog eye lens. Although it could be proven that the gene occurs as a single copy in the haploid yeast genome, replacement of the intact by a disrupted, nonfunctional allele led to no obvious phenotype, indicating that the gene is dispensable. The gene was assigned to chromosome XV. It is transcribed in vivo into an mRNA of about 1300 bases with a coding capacity for a protein of 312 amino acids (estimated M r 35000).