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Stimulation of transcription from accurate initiation sites by purified S‐II
Author(s) -
Hirai Hiroshi,
Sekimizu Kazuhisa,
Horikoshi Masami,
Nakanishi Yoshinobu,
Natori Shunji
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80239-4
Subject(s) - dephosphorylation , transcription (linguistics) , phosphorylation , transcription factor , stimulation , chemistry , transcription factor ii f , microbiology and biotechnology , transcription factor ii e , gene , biology , biochemistry , transcriptional regulation , phosphatase , neuroscience , linguistics , philosophy
The effects of transcription factors S‐II and S‐II′, a phosphorylated form of S‐II, on accurate transcription were compared in a reconstituted transcription system greatly depleted of S‐II. S‐II, but not S‐II′, stimulated the syntheses of run‐off products of various truncated class II genes in this system, suggesting that the activity of this factor is regulated by its phosphorylation and dephosphorylation.