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Mutagenesis directed by phosphotriester analogues of oligonucleotides: A way to site‐specific mutagenesis in vivo
Author(s) -
Petrenko V.A.,
Kipriyanov S.M.,
Boldyrev A.N.,
Pozdnyakov P.I.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80236-9
Subject(s) - site directed mutagenesis , mutagenesis , oligonucleotide , mutant , primer (cosmetics) , directed mutagenesis , biochemistry , in vivo , microbiology and biotechnology , chemistry , gene , biology , genetics , organic chemistry
A new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site‐specific mutagenic primers. The achieved yield of mutant clones was 2–3%.