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Purification and partial characterization of two lectins from the cactus Machaerocereus eruca
Author(s) -
Zenteno Edgar,
Debray Henri,
Montreuil Jean
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80233-3
Subject(s) - eruca , cactus , chemistry , characterization (materials science) , biology , biochemistry , botany , nanotechnology , materials science
Two lectins (MEA I and MEA II ) were isolated from the cactus Machaerocereus eruca by affinity chromatography on mucin‐Sepharose and partially characterized with respect to their biochemical and carbohydrate binding properties. Both are oligomeric glycoproteins consisting of 35 kDa monomers. Amino acid analysis indicates that both lectins have similar composition with high amounts of glycine, glutamic acid and serine. MEA I and MEA II contain ∼ 36 and 24% (w/w) of carbohydrates, respectively. They agglutinate erythrocytes from several animal species. Binding specificity was directed to galactose‐containing oligosaccharides and glycopeptides. The M. eruca lectins are the first lectins to be isolated from a species belonging to the plant family of Cactaceae.