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Regulation by Ca 2+ of membrane elasticity of bovine chromaffin granules
Author(s) -
Miyamoto Shigeaki,
Fujime Satoru
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80226-6
Subject(s) - biophysics , hepes , membrane , exocytosis , chemistry , elasticity (physics) , granule (geology) , swelling , biochemistry , materials science , biology , composite material
In a range of [Ca 2+ ] similar to cytosolic transient, a drastic reduction from about 20 dyn/cm to almost zero was observed in the membrane elastic modulus of bovine chromaffin granules, isolated in a solution containing 0.3 M sucrose and 5 mM Hepes at pH 7.0, and measured by combination of osmotic swelling and dynamic light‐scattering (DLS) methods. This result suggests that the granule membrane becomes extremely flexible as a prelude to exocytosis.