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Nucleotide sequence of cDNA clones encoding the entire precursor polypeptides for subunits IV and V of the photosystem I reaction center from spinach
Author(s) -
Steppuhn J.,
Hermans J.,
Nechushtai R.,
Ljungberg Ulf,
Thümmler F.,
Lottspeich F.,
Herrmann R.G.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80205-9
Subject(s) - spinach , complementary dna , photosystem ii , photosynthetic reaction centre , nucleic acid sequence , nucleotide , sequence (biology) , biology , microbiology and biotechnology , photosystem i , genetics , chemistry , gene , biochemistry , photosynthesis
Using λgt11 expression cloning and immunoscreening, cDNA‐containing recombinant phages for subunits IV and V of the photosystem I reaction center were isolated, sequenced and used to probe Northern blots of polyadenylated RNA prepared from spinach seedlings. The mRNA sizes for both components are ∼ 1000 and 850 nucleotides, respectively. The 968 nucleotide cDNA sequence and derived amino acid sequence for subunit IV predict a single open reading frame of 231 amino acid residues (25.4 kDa). Comparison with a 13‐residue N‐terminal amino acid sequence determined for subunit IV suggests a mature protein of 17.3 kDa (154 residues) and a transit sequence of 77 amino acids (8.1 kDa). The corresponding data for subunit V are 677 bp (cDNA), 167 residues for the precursor protein (18.2 kDa), 98 residues for the mature polypeptide (10.8 kDa) and 69 residues for the transit peptide (7.4 kDa). Secondary structure predictions indicate that both proteins possess greatly different transit sequences and that none is membrane‐spanning.