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Primary and post‐irradiation inactivation of the sulfhydryl enzyme malate synthase: correlation of protective effects of additives
Author(s) -
Durchschlag Helmut,
Zipper Peter
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80203-5
Subject(s) - malate synthase , chemistry , enzyme , primary (astronomy) , atp synthase , biochemistry , citrate synthase , irradiation , malate dehydrogenase , glyoxylate cycle , physics , astronomy , nuclear physics , isocitrate lyase
The presence of additives during X‐irradiation of malate synthase led to radioprotective effects against primary and post‐irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and specific protection are responsible for the protective efficiency of additives. Scavengers delete noxious species formed during irradiation or post‐radiationem. Sulfhydryl reagents may act as repair substances. Specific ligands protect the active site of the enzyme and the essential sulfhydryls; specific protection is more pronounced post‐radiationem. Ligands and sulfhydryl reagents may additionally act as scavengers. A cumulative index for the protective power of additives against both sorts of inactivation was established.