Premium
An antiserum to the C‐terminus of the Alzheimer amyloid precursor recognizes a soluble 70 kDa protein
Author(s) -
Simpson James,
Bladon Christine M.,
Yates Celia M.,
Harmar Anthony J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80200-x
Subject(s) - antiserum , amyloid precursor protein , blot , amyloid (mycology) , microbiology and biotechnology , biochemistry , chemistry , tris , antibody , biology , alzheimer's disease , medicine , immunology , gene , inorganic chemistry , disease
A rabbit antiserum to the C‐terminus of the putative brain amyloid precursor was used to probe Western blots of tissue proteins separated by SDS‐PAGE. The antiserum specifically labelled a protein of approx. 70 kDa in the Tris buffer‐soluble fraction of brain samples from rat, Alzheimer subjects, cases of young and old Down's syndrome, and age‐matched controls. The 70 kDa protein was present in low concentrations in human liver and kidney, and was undetectable in human skeletal muscle. The 70 kDa protein may be a metabolite of the amyloid precursor.