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Two changes of the same nucleotide confer resistance to diuron and antimycin in the mitochondrial cytochrome b gene of Schizosaccharomyces pombe
Author(s) -
Weber S.,
Wolf K.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80165-0
Subject(s) - schizosaccharomyces pombe , antimycin a , schizosaccharomyces , gene , nucleotide , genetics , cytochrome b , mitochondrial dna , biology , mitochondrion , cytochrome , chemistry , biochemistry , saccharomyces cerevisiae , enzyme
Diuron (3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea) and antimycin, both inhibitors of mitochondrial respiration, block electron flow between cytochromes b and c 1 . Mutants resistant to either drug have been selected using Schizosaccharomyces pombe strains with an extrachromosomally inherited mutator. In analogy to Saccharomyces cerevisiae these mutational sites were assumed to map in the cytochrome b gene. DNA sequence analysis showed that two changes in the same nucleotide are responsible for resistance to antimycin and diuron. Analysis of resistant and sensitive progeny of crosses between the mutants and the wild type confirmed the correlation between mutational alteration and resistant phenotype.