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Identification of nucleus‐encoded F 0 I protein of bovine heart mitochondrial H + ‐ATPase as a functional part of the F 0 moiety
Author(s) -
Zanotti Franco,
Guerrieri Ferruccio,
Capozza Guiseppe,
Houštěk Josef,
Ronchi Severino,
Papa Sergio
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80161-3
Subject(s) - cleavage (geology) , nucleus , chemistry , moiety , biochemistry , stereochemistry , crystallography , biology , microbiology and biotechnology , paleontology , fracture (geology)
The F 0 I protein of apparent M r 27000, previously characterized [(1988) Eur. J. Biochem. 173, 1–8] as a genuine component of bovine heart F 0 , has been sequenced and shown to be identical with the nucleus encoded 24668 Da protein characterized earlier [(1987) J. Mol. Biol. 197, 89–100]. It is directly shown by proteolytic cleavage and reconstitution experiments that this protein, denoted here as PVP from the single‐letter codes of the last three residues of the N‐terminus, is involved in proton conduction by F 0 and in its sensitivity to oligomycin.