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The amino‐terminal structure of oat phytochrome
Author(s) -
Grimm Rudolf,
Kellermann Josef,
Schäfer Wolfram,
Rüdiger Wolfhart
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80146-7
Subject(s) - phytochrome , etiolation , trypsin , biochemistry , chemistry , amino acid , peptide sequence , serine , biology , enzyme , botany , gene , red light
Phytochrome from etiolated oat seedlings was digested under carefully controlled conditions with trypsin. A dodecapeptide which contains the blocked amino‐terminus of phytochrome was isolated from the digestion mixture and analyzed. According to amino acid analysis and the results obtained in FAB mass spectrometry, the sequence of mature phytochrome starts with N ‐acetylserine, corresponding to serine‐2 derived from the DNA sequence.