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The Kluyveromyces lactis KEX1 gene encodes a subtilisin‐type serine proteinase
Author(s) -
Tanguy-Rougeau Christine,
Wésolowski-Louvel Micheline,
Fukuhara Hiroshi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80139-x
Subject(s) - subtilisin , kluyveromyces lactis , chemistry , serine , biochemistry , kluyveromyces , gene , microbiology and biotechnology , biology , enzyme , saccharomyces cerevisiae
KEX1 is a chromosomal gene required for the production of the killer toxin encoded by the linear DNA plasmid pGKL‐1 of Kluyveromyces lactis . The nucleotide sequence of the cloned KEX1 gene has been determined. The deduced structure of the KEX1 protein, 700 amino acids long, indicated that it contained an internal domain with a striking homology to the sequences of the subtilisin‐type proteinases, and a probable transmembrane domain near the carboxyl terminus. The results confirm the hypothesis that the product of the gene KEX1 of K. lactis is a proteinase involved in the processing of the toxin precursor.