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Increased membrane heterogeneity in stimulated human granulocytes
Author(s) -
Valentino Matteo,
Governa Mario,
Gratton Enrico,
Fiorini Rosamaria,
Curatola Giovanna,
Bertoli Enrico
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80136-4
Subject(s) - fluorescence , membrane , biophysics , chemistry , stimulation , redistribution (election) , fluorescence spectroscopy , analytical chemistry (journal) , chromatography , biochemistry , biology , physics , optics , neuroscience , politics , political science , law
TMA‐DPH fluorescence decay in human PMN before and after stimulation with FMLP was studied using frequency domain fluorometry. Membrane heterogeneity was assessed by the width of the continuous distributions of lifetime values of Lorentzian shape used to describe the fluorescence decay. In non‐stimulated granulocytes TMA‐DPH fluorescence decay is characterized by two distributions of lifetime values centered at 6.5 and 1.0 ns and full width at half maximum of 0.3 and 1.2 ns, respectively. Within 15 min after stimulation, the center values of the two distribution components were 5.1 and 0.8 ns and the distribution width was 0.8 and 0.6 ns, respectively. These results indicate changes of membrane domain organization which can be ascribed to compositional changes redistribution of membrane components.