Premium
Gene cloning from yeast chromosome‐specific mini‐library Isolation of the SRP1 ‐related DNA sequence located on chromosome XV
Author(s) -
Marguet D.,
Fantino E.,
Lauquin G.J.-M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80129-7
Subject(s) - genetics , cloning (programming) , chromosome , biology , gene , yeast artificial chromosome , sequence (biology) , isolation (microbiology) , dna , dna sequencing , chromosome 21 , chromosome 22 , microbiology and biotechnology , gene mapping , bioinformatics , computer science , programming language
We have isolated and purified chromosome XV DNA molecules from the yeast S. cerevisiae using contour‐clamped homogeneous electric field (CHEF) gel electrophoresis. A chromosome‐specific mini‐library was constructed and the element of the SRP1 (serine‐rich protein) related sequence family located within chromosome XV was isolated by in situ colony hybridization with an SRP1 probe. Results indicated that (i) a single‐copy sequence homologous to SRP1 is present within chromosome XV; (ii) this sequence lies within the 2.3 kb Hind III fragment of the plasmid pXVAc6; (iii) the lack of a specific transcript from this SRP1 ‐related element suggests that it could be considered as a pseudogene.