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Quantification of DNA‐dependent RNA polymerase subunits and initiation factor(s) by antibody‐linked polymerase assays
Author(s) -
Lerbs-Mache S.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80123-6
Subject(s) - polymerase , microbiology and biotechnology , nitrocellulose , dna polymerase , rna polymerase , chemistry , enzyme , biochemistry , biology , real time polymerase chain reaction , rna polymerase ii , rna , membrane , gene , gene expression , promoter
The antibody‐linked polymerase assay is a method which allows one to assign RNA polymerase activity to SDS‐denatured polypeptides on nitrocellulose membranes using antibodies which were raised against only partially purified polymerase preparations. Here we show that with this method not only enzyme subunits but also initiation factor(s) can be determined in crude homogenates. Moreover the determination is quantitative. Therefore changes in the amount of individual polymerase subunits and factor(s) can be visualized within different crude homogenates.