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Isolation of protein kinase C subspecies from a preparation of human T lymphocytes
Author(s) -
Shearman Mark S.,
Berry Nicola,
Oda Tomiichiro,
Ase Katsuhiko,
Kikkawa Ushio,
Nishizuka Yasutomi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80122-4
Subject(s) - subspecies , protein kinase c , biology , enzyme , microbiology and biotechnology , t lymphocyte , biochemistry , beta (programming language) , protein kinase a , chemistry , in vitro , paleontology , computer science , programming language
Using a preparation of purified human T lymphocytes, we were able to resolve a partially purified protein kinase C (PKC) enzyme fraction into two distinct subspecies, of approximately equal activity. Biochemical and immunocytochemical analysis revealed that these fractions closely resembled the type II(β) and type III(α) PKC subspecies previously identified and characterised from brain tissue. These results provide valuable information for further studies on the role of individual PKC subspecies in T lymphocyte proliferation.