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An increase in intracellular pH is a general response of promonocytic cells to differentiating agents
Author(s) -
Ladoux Annie,
Damais Chantal,
Krawice Irène,
Abita Jean Pierre,
Frelin Christian
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80115-7
Subject(s) - retinoic acid , intracellular , butyrate , monocyte , intracellular ph , u937 cell , chemistry , hl60 , interferon , microbiology and biotechnology , biochemistry , biology , immunology , in vitro , fermentation , gene
Intracellular pH (pHi) was measured in HL60 and U937 cells before and after differentiation into monocyte‐macrophage like cells. 12‐ O ‐Tetradecanoyl phorbol‐13‐acetate (PMA), butyrate, interferon, retinoic acid and 1,25‐dihydroxyvitamin D 3 all increased pHi. The increases elicited were rapid with PMA, much slower with retinoic acid and interferon and still slower with 1,25‐dihydroxyvitamin D 3 . Increases in pHi are due to an activation of the Na + /H + exchange system. High pHi values are unlikely to serve as an early intracellular signal for initiating monocytic differentiation.