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Guanine nucleotide‐induced Ca 2+ release in permeabilized murine thymocytes
Author(s) -
Ueda Teruko,
Ichikawa Yoichi,
Kusaka Iwao
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80096-6
Subject(s) - gtp' , nucleotide , chemistry , guanine , biophysics , biochemistry , microbiology and biotechnology , biology , enzyme , gene
GTP and IP 3 induced Ca 2+ release from an internal store in permeabilized murine thymocytes loaded with Ca 2+ by ATP. Ca 2+ release was dependent on the concentration of GTP: half‐maximal release with 0.5 μM and maximal release with 10 μM. The GTP effect was completely abolished by 100 μM GTPγS, GMPPNP and UTP. None of the other nucleotides used except ITP induced Ca 2+ release. When GTP was added after the effect of IP 3 had virtually subsided, and vice versa, further Ca 2+ release occurred, which led to the conclusion that the mechanism of GTP‐mediated Ca 2+ release may be different from that of IP 3 ‐mediated release.