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Pertussis toxin‐dependent and ‐independent hormonal effects on cultured renal epithelioid cells
Author(s) -
Paulmichl M.,
Wöll E.,
Lang F.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80094-2
Subject(s) - pertussis toxin , bradykinin , medicine , g protein , endocrinology , acetylcholine , potassium channel , epinephrine , serotonin , toxin , chemistry , biology , microbiology and biotechnology , biochemistry , signal transduction , receptor
The present study has been performed to test for the involvement of pertussis toxin‐sensitive GTP‐binding proteins (G‐proteins) in the cellular transduction of hormone‐induced activation of potassium channels. In Madin Darby canine kidney (MDCK) cells, a permanent cell line from dog kidney, epinephrine, acetylcholine, bradykinin, serotonin and ATP hyperpolarize the cell membrane by activation of potassium channels. In cells pretreated with pertussis toxin the hyperpolarizations elicited by either acetylcholine or serotonin are completely abolished; that following epinephrine is blunted and only transient. The hyperpolarizing effects of ATP or bradykinin are not affected by pertussis toxin. Thus, in MDCK cells both pertussis toxin‐dependent and ‐independent mechanisms operate in parallel to enhance the potassium conductance of the cell membrane.