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Studies on the import into mitochondria of yeast ATP synthase subunits 8 and 9 encoded by artificial nuclear genes
Author(s) -
Law Ruby H.P.,
Farrell Leigh B.,
Nero Debra,
Devenish Rodney J.,
Nagley Phillip
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80086-3
Subject(s) - neurospora crassa , protein subunit , mitochondrion , saccharomyces cerevisiae , biology , nuclear gene , biochemistry , mutant , atp synthase , yeast , gamma aminobutyric acid receptor subunit alpha 1 , fusion protein , gene , microbiology and biotechnology , mitochondrial dna , g alpha subunit , recombinant dna
Direct fusions have been constructed between each of subunits 8 and 9 from mitochondrial ATPase of Saccharomyces cerevisiae , proteins normally encoded inside mitochondria, and the cleavable N‐terminal transit peptide from the nuclearly encoded precursor to subunit 9 of Neurospora crassa mitochondrial ATPase. The subunit 8 construct was imported efficiently into isolated yeast mitochondria and was processed at or very near the fusion point. When expressed in vivo from its artificial nuclear gene, this cytoplasmically synthesized form of subunit 8 restored the growth defects of aap1 mutants unable to produce subunit 8 inside the mitochondria. The subunit 9 construct was, however, unable to be imported into isolated mitochondria and could not, following nuclear expression in vivo, complement growth defects in mitochondrial oli1 mutants. This behaviour is contrasted with the previously demonstrated import competence of another yeast subunit 9 fusion, bearing the first five residues of mature N. crassa subunit 9 interposed between its own transit peptide and the yeast subunit 9 moiety.