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Porcine brain natriuretic peptide, another modulator of bovine adrenocortical steroidogenesis
Author(s) -
Hashiguchi Takashi,
Higuchi Kazumi,
Ohashi Masao,
Minamino Naoto,
Kangawa Kenji,
Matsuo Hisayuki,
Nawata Hajime
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80076-0
Subject(s) - adrenal cortex , medicine , endocrinology , angiotensin ii , receptor , atrial natriuretic peptide , aldosterone , binding site , brain natriuretic peptide , peptide , in vitro , chemistry , biology , biochemistry , heart failure
Porcine brain natriuretic peptide (pBNP) significantly inhibited aldosterone production stimulated by an angiotensin II analog and ACTH‐stimulated cortisol secretion, together with simultaneously increasing the formation of cGMP in dispersed bovine adrenocortical cells. Receptors for pBNP were identified in bovine adrenal gland using an in vitro receptor autoradiographic technique and studies of 125 I‐pBNP binding. In vitro receptor autoradiography demonstrated specific binding sites for 125 I‐pBNP in bovine adrenal cortex. Complete displacement of 125 I‐pBNP by unlabeled pBNP or human atrial natriuretic peptide (hANP) can take place at these sites. Analysis of 125 I‐pBNP binding to bovine adrenocortical membrane fractions showed that the adrenal cortex had high‐affinity, low‐capacity pBNP‐binding sites, with a dissociation constant ( K d ) of 2.32 ± 0.33 × 10 −10 M (mean ± SE) and a maximal binding capacity ( B max ) of 36.7 ± 1.6 fmol/mg protein. Moreover, the specific binding sites for 125 I‐pBNP were completely displaced not only by unlabeled pBNP but also by unlabeled hANp. The hANP dose required for 50% inhibition of specific 125 I‐pBNP binding was almost identical to that for pBNP (IC 50 values for hANP and pBNP: 8.5 × 10 −10 and 6.5 × 10 −10 M, respectively). These results suggest that pBNP exerts a suppressive effect on bovine adrenocortical steroidogenesis via a receptor which may be shared with ANP.